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. Author manuscript; available in PMC: 2023 Jul 20.
Published in final edited form as: Nature. 2022 Jun 22;607(7917):142–148. doi: 10.1038/s41586-022-04866-z

Extended Data Figure 2. Nfil3 acts as a repressor to drive cDC1 specification.

Extended Data Figure 2.

a, Gating strategy for MDP, CDP and pre-cDC1 progenitors in BM. b, Flow cytometric analysis showing pDCs and cDCs differentiated from GFP negative and positive CDPs sorted from Nfil3GFP/GFP mice. Data shown are one of four similar experiments. c, Flow cytometric analysis showing pDCs and cDCs differentiated from WT CD117hi BM progenitors retrovirally expressing NFIL3, NFIL3-KRAB or NFIL3-VP16. The cDCs are pre-gated as CD317− B220− MHCII+ CD11c+ cells. Data shown are one of three similar experiments. Schematic diagrams of NFIL3, NFIL3-KRAB and NFIL3-VP16 are depicted below the plots. DBD denotes DNA-binding domain.