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. Author manuscript; available in PMC: 2023 Jul 20.
Published in final edited form as: Nature. 2022 Jun 22;607(7917):142–148. doi: 10.1038/s41586-022-04866-z

Extended Data Figure 5. Mutation of three NFIL3 binding sites in the −165 kb Zeb2 enhancer abrogates cDC2 development.

Extended Data Figure 5.

a, Representative flow plots showing cDCs and pDCs among live splenocytes from WT, Δ1, Δ1+2, Δ1+3 and Δ1+2+3 mice. cDCs are pre-gated as CD317− B220− MHCII+ CD11c+ cells. b, Number of splenic cDC1, cDC2, pDC, and frequency of splenic pDC in WT, Δ1, Δ1+2, Δ1+3 and Δ1+2+3 mice. Data are pooled from five independent experiments (n = 12 for WT, n = 11 for Δ1, Δ1+2, Δ1+3, and n = 8 for Δ1+2+3 mice). NS, not significant; b: Brown–Forsythe and Welch ANOVA with Dunnetťs T3 multiple comparisons test.