Figure 6.

Quantification of the abundance of WRN peptides containing C727 in DMSO controls versus samples treated with a 10-fold excess of H3B-219 (left) or H3B-960 (right) reveals significant depletion of the peptides treated with compound under multiple proteolytic conditions. Total protein quantification (representative example for samples treated with chymotrypsin shown) indicated that the depletion was not due to differences in sample concentration, suggesting that peptides containing C727 become alkylated upon compound treatment.