TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype and/or characteristics | Source or reference |
|---|---|---|
| B. subtilis strains | ||
| IS75 | his leu met | Laboratory strain |
| BD1853 | comPΔBclI orf2::kan | Laboratory strain |
| BD1890 | srfA-lacZ | 23 |
| BD2356 | comXP::spc | This work |
| BD2358 | comXP::spc carrying plasmid Pspac comP | This work |
| BD2362 | comXP::spc carrying plasmid Pspac | This work |
| BD2744 | amyE::ΔLabc comP comXP::spc | This work |
| BD2745 | amyE::ΔLbc comP comXP::spc | This work |
| BD2746 | amyE::ΔLc comP comXP::spc | This work |
| BD2747 | amyE::Xba comP comXP::spc | This work |
| BD2757 | amyE::ΔLabc comP comP ΔBclI | This work |
| BD2758 | amyE::ΔLbc comP comP ΔBclI | This work |
| BD2759 | amyE::ΔLc comP comP ΔBclI | This work |
| BD2760 | amyE::XbaI comP comP ΔBclI | This work |
| Plasmidsa | ||
| Pspac | B. subtilis vector | |
| Pspac comP | Pspac plasmid carrying comP | This work |
| pUCCMPHOA | Integrative vector for construction of in-frame fusions to the phoA gene of E. coli | 21 |
| pAH (H) | comP (D106)b cloned in pUCCMPHOA | This work |
| pAF (F) | comP (R112) cloned in pUCCMPHOA | This work |
| pAI (I) | comP (R143) cloned in pUCCMPHOA | This work |
| pAL (L) | comP (E223) cloned in pUCCMPHOA | This work |
| pAM (M) | comP (K272) cloned in pUCCMPHOA | This work |
| pAN (N) | comP (Y299) cloned in pUCCMPHOA | This work |
| pAZ (Z) | comP (P314) cloned in pUCCMPHOA | This work |
| pAO (O) | comP (Y338) cloned in pUCCMPHOA | This work |
| pAP (P) | comP (I362) cloned in pUCCMPHOA | This work |
| pAG (G) | comP (F396) cloned in pUCCMPHOA | This work |
| pAY (Y) | comP (L500) cloned in pUCCMPHOA | This work |
| JF751 | Integrative vector for construction of in-frame fusions to lacZ | 46 |
| pQR (R) | comP (K272) cloned in JF751 | This work |
| pQS (S) | comP (Y299) cloned in JF751 | This work |
| pQW (W) | comP (P314) cloned in JF751 | This work |
| pQT (T) | comP (Y338) cloned in JF751 | This work |
| pQU (U) | comP (I362) cloned in JF751 | This work |
| pQV (V) | comP (F396) cloned in JF751 | This work |
| pQX (X) | comP (L500) cloned in JF751 | This work |
a
The letters in parentheses designate the fusion proteins encoded by the plasmid.
b
The last aminoacyl residue of ComP included in the fusion is given in parentheses.