TABLE 1.
Strain or plasmid | Relevant genotype and/or characteristics | Source or reference |
---|---|---|
B. subtilis strains | ||
IS75 | his leu met | Laboratory strain |
BD1853 | comPΔBclI orf2::kan | Laboratory strain |
BD1890 | srfA-lacZ | 23 |
BD2356 | comXP::spc | This work |
BD2358 | comXP::spc carrying plasmid Pspac comP | This work |
BD2362 | comXP::spc carrying plasmid Pspac | This work |
BD2744 | amyE::ΔLabc comP comXP::spc | This work |
BD2745 | amyE::ΔLbc comP comXP::spc | This work |
BD2746 | amyE::ΔLc comP comXP::spc | This work |
BD2747 | amyE::Xba comP comXP::spc | This work |
BD2757 | amyE::ΔLabc comP comP ΔBclI | This work |
BD2758 | amyE::ΔLbc comP comP ΔBclI | This work |
BD2759 | amyE::ΔLc comP comP ΔBclI | This work |
BD2760 | amyE::XbaI comP comP ΔBclI | This work |
Plasmidsa | ||
Pspac | B. subtilis vector | |
Pspac comP | Pspac plasmid carrying comP | This work |
pUCCMPHOA | Integrative vector for construction of in-frame fusions to the phoA gene of E. coli | 21 |
pAH (H) | comP (D106)b cloned in pUCCMPHOA | This work |
pAF (F) | comP (R112) cloned in pUCCMPHOA | This work |
pAI (I) | comP (R143) cloned in pUCCMPHOA | This work |
pAL (L) | comP (E223) cloned in pUCCMPHOA | This work |
pAM (M) | comP (K272) cloned in pUCCMPHOA | This work |
pAN (N) | comP (Y299) cloned in pUCCMPHOA | This work |
pAZ (Z) | comP (P314) cloned in pUCCMPHOA | This work |
pAO (O) | comP (Y338) cloned in pUCCMPHOA | This work |
pAP (P) | comP (I362) cloned in pUCCMPHOA | This work |
pAG (G) | comP (F396) cloned in pUCCMPHOA | This work |
pAY (Y) | comP (L500) cloned in pUCCMPHOA | This work |
JF751 | Integrative vector for construction of in-frame fusions to lacZ | 46 |
pQR (R) | comP (K272) cloned in JF751 | This work |
pQS (S) | comP (Y299) cloned in JF751 | This work |
pQW (W) | comP (P314) cloned in JF751 | This work |
pQT (T) | comP (Y338) cloned in JF751 | This work |
pQU (U) | comP (I362) cloned in JF751 | This work |
pQV (V) | comP (F396) cloned in JF751 | This work |
pQX (X) | comP (L500) cloned in JF751 | This work |
The letters in parentheses designate the fusion proteins encoded by the plasmid.
The last aminoacyl residue of ComP included in the fusion is given in parentheses.