TABLE 2.
Effect of metabolic inhibitors on nickel transporta
| Inhibitor | % of Ni transportb |
|---|---|
| Control (no addition) | 100 |
| ATPase inhibitors | |
| DCCD (200 μM) | 220 |
| Oligomycin (50 μg/ml) | 142 |
| Electron sink/transport | |
| Sodium azide (1,000 μM) | 75 |
| Potassium cyanide (200 μM) | 0 |
| Hydroxyl amine (1,000 μM) | 67 |
| Protonophores | |
| CCCP (200 μM) | 56 |
| DNP (200 μM) | 74 |
| Metal ionophores | |
| Valinomycin (50 μg/ml) | 109 |
| Valinomycin (50 μg/ml) plus 50 mM KCl | 154 |
| Nigericin (50 μg/ml) | 96 |
| Gramicidin D (50 μg/ml) | 165 |
| Miscellaneous | |
| COc | 102 |
| O2d | 48 |
| Darke | 187 |
| Cold (4°C) | 50 |
a
Transport assays were performed as described in Materials and Methods. The indicated inhibitor was added to the cells and incubated for 30 min in a shaking 30°C water bath with illumination. Ni2+ was added to a final concentration of 1.5 μM, and then the sample was assayed as described in the text.
b
Percentage of control (100% = 25 pmol of Ni2+/min/mg of protein).
c
Gas phase was 20% CO and 80% N2.
d
Gas phase was air.
e
Vials containing cells were covered with aluminum foil.