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. 1999 Aug;181(15):4568–4575. doi: 10.1128/jb.181.15.4568-4575.1999

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Copyright © 1999, American Society for Microbiology

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FIG. 2 — SDS-PAGE of overexpressed Are proteins. The lanes contain lysates from E. coli BL21(DE3)pLysS (lane 2) on carrying the following plasmids: pET5a (lane 3), pADPW40 (lane 4), pADPW40 (lane 5), pADPW30 (lane 6), pADPW30 (lane 7), pADPW29 (lane 8), or pADPW29 (lane 9). The proteins were induced (lanes 5, 7, and 9) or not induced (lanes 4, 6, and 8) with IPTG. Lanes 1 and 10 contain the following molecular mass standards (values in kilodaltons): A, 97.4; B, 66.2; C, 45.0; D, 31.0; E, 21.5; and F, 14.5. The estimated molecular masses for the overexpressed bands are as follows: AreA (lane 5), 37 kDa; AreB (lane 7), 39 kDa with minor components at 32 and 27 kDa; and AreC (lane 9), 52 kDa.