TABLE 3.
Relative activities of AreC BZDH against different benzaldehydes in crude extracts of cells grown on different substrates
| Strain | Growth substrate | Relative activitya (sp act [U/mg of protein]) against benzaldehyde with substituent:
|
||||||
|---|---|---|---|---|---|---|---|---|
| None | 2-Methyl | 3-Methyl | 4-Methyl | 2-OH | 3-OH | 4-OH | ||
| E. coli (pADPW29) | LB + Ampb + IPTG | 100 (3.0) | <1 | 67 | 29 | 600 | 102 | 80 |
| ADP1 | Benzyl alcohol | 100 (0.29) | <1 | 80 | 33 | 600 | 101 | 117 |
| ADP1 | Benzyl acetate | 100 (0.16) | <1 | 80 | 29 | 600 | 101 | 96 |
| ADP1 | 4-OH benzyl alcohol | 100 (0.27) | <1 | 80 | 28 | 600 | 105 | 107 |
| ADP1 | Succinate | (<0.001) | ND | ND | ND | ND | ND | ND |
a
Activity relative to benzaldehyde, which is set at 100. All reaction rates upon which the relative activities were based were the averages of three measurements, none of which varied by >5%. ND, not determined.
b
Amp, ampicillin.