TABLE 4.
Relative activities of AreB BADH against various benzyl alcohols in crude extracts of cells grown on different substrates
| Strain | Growth substrate | Relative activitya (sp act [U/mg of protein]) against benzyl alcohol with substituent:
|
||||||
|---|---|---|---|---|---|---|---|---|
| None | 2-Methyl | 3-Methyl | 4-Methyl | 2-OH | 3-OH | 4-OH | ||
| E. coli (pADPW30) | LB + Ampb + IPTG | 100 (2.3) | 78 | 105 | 107 | 47 | 41 | 18 |
| ADP1 | Benzyl alcohol | 100 (0.94) | 62 | 99 | 97 | 42 | 32 | 14 |
| ADP1 | Benzyl acetate | 100 (0.90) | 63 | 103 | 106 | 43 | 33 | 11 |
| ADP1 | 4-OH benzyl alcohol | 100 (0.92) | 57 | 106 | 110 | 50 | 51 | 14 |
| ADP1 | Succinate | (<0.001) | ND | ND | ND | ND | ND | ND |
a
Activity relative to benzyl alcohol, which is set at 100. All reaction rates upon which the relative activities are based were the averages of three measurements none of which varied by >5%. ND, not determined.
b
Amp, ampicillin.