TABLE 5.
Kinetic parameters for AreA benzyl esterase
| Substrate | Km (μM) (range)a | Vmax (μmol/min/mg) (range)a | Vmax/Km |
|---|---|---|---|
| 4-Nitrophenyl acetate | 2,410 (2,260–2,720) | 220 (207–236) | 0.11 |
| 4-Nitrophenyl butyrate | 61 (53–68) | 960 (912–1,020) | 16 |
| 4-Nitrophenyl hexanoate | 11 (10.7–12.3) | 430 (424–443) | 39 |
| Benzyl acetate | 227 (213–238) | 690 (670–712) | 3 |
| Benzyl propionate | 67 (63–70) | 2,090 (2,020–2,140) | 31 |
| Benzyl butyrate | 42.4 (40–45) | 2,050 (2,010–2,090) | 47 |
| Benzyl benzoate | 47 (43.6–49) | 1,100 (1,070–1,130) | 23 |
a
The range in parentheses after each value specifies the 68% confidence limits.