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. 2023 Jul 6;14:1114211. doi: 10.3389/fendo.2023.1114211

Figure 3.

Figure 3

DEX-treatment had no overall effect on gene expression levels in ex vivo cultured human fetal adrenals. Gene expression levels of (A) transcription factors (SF-1 and DAX-1), (B) receptors (MC2R and GR) and (C) enzymes important for adrenal steroidogenesis (StAR, CYP11A1, CYP17A1, 3βHSD2, CYP21A2, CYP11B1/2 and SULT2A1) examined in ex vivo cultured HFA tissue cultured with DEX (10 μM) for 14 days (Experimental setup A). Tissue from 1-11 fragments were pooled per sample and treatment depending on the initial size of half of the adrenal gland and quantitative reverse transcription polymerase chain reaction analysis was performed. Gene expression is relative to the reference gene RPS20 and normalized to the mean of vehicle controls. Values are shown as geometric mean with 95% CI, n = 10 fetuses. Significant differences between DEX treated and vehicle controls from the same fetus were based on ln-transformed data using paired t-test.