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. 2023 Jul 6;14:1170035. doi: 10.3389/fimmu.2023.1170035

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Copyright © 2023 Macchia, La Sorsa, Urbani, Moretti, Antonucci, Afferni and Schiavoni

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation for principal methods of isolation and study of eosinophils from peripheral blood. Whole blood samples can be either directely stimulated in culture and stained with a multicolor panel to distinguish eosinophils from other leukocytes, such as neutrophils and basophils, based on indicated surface markers. Alternatively, eosinophils can be isolated from whole blood by density gradient enrichment followed by immunomagnetic or cell sorting purification. Eosinophils can be then assessed for phenotype (by expression of indicated activation markers) and function, either ex vivo or following in vitro stimulation.