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Determination of the transcription start site of yaaH by primer extension analysis. RNA prepared from sporulating cells of strain NIS8114 was hybridized with primers RT2 (A) and RT1 (B). Lanes labeled A, G, C, and T are DNA sequencing reactions with appropriate primers. Primer extension products are marked with arrowheads, and the transcription start site on the yaaH upstream sequence is marked with an asterisk and a capital letter.
