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Detection of YaaH-His in mature spores. Spore proteins were solubilized from B. subtilis 168 transformants carrying control vector pTUBE1200H6 (lane 1) or pYAAH8 (lane 2) as described in Materials and Methods. The proteins were resolved by SDS-PAGE (15% acrylamide gel) and visualized by Coomassie brilliant blue staining (A) or immunoblotting with anti-His tag antiserum (B). Arrowheads indicate the deduced molecular mass of the YaaH-His protein.
