TABLE 2.
Heat resistance of spores after incubation in the presence and absence of germinantsa
| Genotype | No. of spores in the presence of:
|
||
|---|---|---|---|
| Tris-HCl buffer | AGFK | l-Alanine | |
| Wild type | 1.5 × 108 | 5.1 × 106 | 9.0 × 104 |
| yaaH mutant | 1.2 × 108 | 6.7 × 106 | 3.0 × 107 |
a
Spores were incubated in 10 mM Tris-HCl buffer (pH 7.0) without or with AGFK or l-alanine at 37°C for 90 min. The samples were heated at 80°C for 30 min and then spread on LB agar medium after appropriate dilution. The proportion of survivors was determined by counting colonies after 12 h of incubation at 37°C. The data represent the averages of three independent experiments.