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. 2023 Jul 10;19(7):e1010519. doi: 10.1371/journal.pgen.1010519

Fig 1. ADAD2 interacts with another RNA binding protein, RNF17.

Fig 1

A. Number of mass spectrometry-identified proteins from ADAD2 immunoprecipitation (IP) of whole testis lysate from 42 dpp wildtype (grey ovals, n = 3) or Adad2M/M (purple oval, n = 1). B. Summary of proteins detected across all wildtype IPs but not Adad2M/M. RNF17, protein of interest, in green, and ADAD2, for confirmation of genotype and IP, in purple. Table inset includes remaining 14 proteins identified in all three mutants but not wildtype. Underline indicates well-known post-meiotic germ cell proteins. C. Confirmation of ADAD2-RNF17 interaction via immunoprecipitation-Western blotting in wildtype, Adad2M/M, and Rnf17M/M 42 dpp whole testis lysates demonstrating a specific interaction between ADAD2 and RNF17L. ‘Input’—total testis lysate, ‘FT’–flow-through (unbound lysate proteins), ‘wash’—first wash of beads post binding, ‘IP’—immunoprecipitate. Blots representative of results from at least three IPs per genotype. Approximate molecular weight reported for each band.