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. 2023 Jul 10;19(7):e1010519. doi: 10.1371/journal.pgen.1010519

Fig 4. ADAD2 and RNF17 colocalize to form multiple distinct populations of granules some of which associate with the IMC.

Fig 4

A. Co-immunofluorescence of ADAD2 and RNF17 in adult wildtype testes across selected pachytene spermatocyte developmental stages demonstrating colocalization. Roman numerals–testis tubule cross-section stage (VIII containing mid-stage pachytene spermatocytes, IX through XI containing late-stage pachytene spermatocytes). Asterisks—small granules and arrowheads—large granules. Red—RNF17, green—ADAD2, and blue—DAPI. Co-immunofluorescence of B. NANOS1 and ADAD2 or RNF17 demonstrating NANOS1 co-localization in both large and small ADAD2 or RNF17 granules (red—NANOS1, green—ADAD2 or RNF17, and blue–DAPI), C. PIWIL1 with ADAD2 or RNF17 in adult wildtype testes demonstrating co-localization in a subset of ADAD2 or RNF17 granules (red—PIWIL2 or PIWIL1, green—ADAD2 or RNF17, and blue–DAPI), and D. the mitochondrial marker COX IV and ADAD2 or RNF17 demonstrating a subset of small granules colocalize with the mitochondria but large granules do not (red–COX IV, green–ADAD2 or RNF17, and blue–DAPI). Asterisks—small ADAD2 or RNF17 granules. Arrowheads—large ADAD2 or RNF17 granules. 630x magnification for all images.