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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Adv Healthc Mater. 2022 Dec 23;12(7):e2202221. doi: 10.1002/adhm.202202221

Figure 5: Neurons form less cellular aggregates on more conductive scaffolds and neurites span the scaffold architecture.

Figure 5:

(A) Photomicrographs of NPCs in the scaffolds of different mechanical properties (viscoelastic, elastic) and scaffold carbon content (%), compared after 6 weeks in culture and stained for neuronal markers. Tuj1 (green), NeuN (white), NPC (red), Hoescht (blue). Scale bar: 310 μm. (B) Quantification of the number of cells that have aggregated together (aggregate diameter > 100 μm) across scaffold carbon content (%) and mechanical properties (viscoelastic: blue; elastic: red). At least 5 field of views compared for each condition. (C) Quantification of the neurons (Tuj1+) in culture (%), across scaffold carbon content (%) and mechanical properties (viscoelastic: blue; elastic: red). At least 7 fields of view compared for each condition. All numerical data are presented as mean ± s.d. (one-way analysis of variance (ANOVA) and Tukey’s honestly significant difference (HSD) post hoc test: ****P < 0.0001, 0.001<**-<0.01, 0.01 < *P < 0.05, non-significant (n.s.) P > 0.05).