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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Adv Healthc Mater. 2022 Dec 23;12(7):e2202221. doi: 10.1002/adhm.202202221

Figure 7: Myelination length and quantity is impacted by scaffold mechanical and electrical properties.

Figure 7:

(A) 3D reconstructions of photomicrographs of NPCs in the scaffolds of different mechanical properties (viscoelastic, elastic) and scaffold carbon content (%), compared after 6 weeks in culture and stained for oligodendrocyte marker. Tuj1 (green), MBP (white-magenta), NPC (red), Hoescht (blue). Scale bar: 180 μm. (B) Photomicrograph of a viscoelastic scaffold with 0.37% carbon content, showing myelinated (white-magenta) neurites (green) spanning the entire length of the scaffold. Scale bar: 240 μm. (C) Quantification of the length of myelin (white-magenta, μm) across scaffold carbon content (%) and mechanical properties (viscoelastic: blue; elastic: red). At least 5 field of views compared for each condition. All numerical data are presented as mean ± s.d. (one-way analysis of variance (ANOVA) and Tukey’s honestly significant difference (HSD) post hoc test: ***P < 0.0001, 0.001 < **P < 0.01, 0.01 < * P < 0.05, non-significant (n.s.) P > 0.05).