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. Author manuscript; available in PMC: 2023 Jul 20.
Published in final edited form as: Sci Transl Med. 2022 Sep 14;14(662):eabq3215. doi: 10.1126/scitranslmed.abq3215

Fig. 3. PAR induces R-DPR condensation in vitro.

Fig. 3.

(A) Co-IP of PAR and DPRs in HEK293T cells. (B) Dot blot binding assays of PAR and DPRs. (C) TAMRA–R-DPR (red) mixed with PAR (10 to 50 μM MAR equivalent) or MAR. (D and E) FRAP assays on PAR and TAMRA–R-DPR (red) condensates. Yellow circles indicate bleached and analyzed areas. Total R-DPRs (1 μM) were labeled. Buffer for (C to E): 61.5 mM K2HPO4 and 38.5 mM KH2PO4