Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6JRj | Janvier Labs | Female mice | |
| Strain, strain background (M. musculus) | B6.129S7-Rag1, C57BL/6JRj |
Jackson Laboratory | IMSR_JAX:002216 | Breeding pairs, homozygous for Rag1 |
| Strain, strain background (M. musculus) | B6.129S2-Ighm, C57BL/6JRj | Jackson Laboratory | IMSR_JAX:002288 | Breeding pairs, homozygous for Ighm |
| Antibody | Anti-human CD19-VioGreen, (clone REA675) (human monoclonal) |
Miltenyi Biotec | AB_2726202 | 1:400 |
| Antibody | Anti-human anti-IgD-VioBlue (clone IgD26) (mouse monoclonal) |
Miltenyi Biotec | AB_2659773 | 1:400 |
| Antibody | Anti-human CD27-APC (clone M-T271) (mouse monoclonal) |
Miltenyi Biotec | AB_1036201 | 1:400 |
| Antibody | Anti-human CD86-PE-Vio770 (clone FM95) (mouse monoclonal) | Miltenyi Biotec | AB_275113 | 1:400 |
| Antibody | Anti-human CD38-FITC (clone IB6) (mouse monoclonal) | Miltenyi Biotec | AB_615091 | 1:400 |
| Antibody | Anti-human anti-IgM-PE (clone PJ2-22H3) (mouse monoclonal) | Miltenyi Biotec | AB_1036088 | 1:400 |
| Antibody | Alkaline phosphatase (AP)-labeled (goat polyclonal) anti-human IgM | μ-chain specific; Sigma-Aldrich | n/a | 1:50,000 in TBS BSA |
| Antibody | Alkaline phosphatase (AP)-labeled (goat polyclonal) anti-human IgG | γ-chain specific; Sigma-Aldrich | n/a | 1:50,000 in TBS BSA |
| Antibody | Alkaline phosphatase (AP)-labeled (goat polyclonal) anti-human IgA | α-chain specific; Sigma-Aldrich | n/a | 1:50,000 in TBS BSA |
| Antibody | Anti-mouse IgD-Pacific Blue (clone 11–26c.2a) (rat monoclonal) | BioLegend | AB_1937245 ( Cat#. 405711); AB_1937244 (Cat# 405712) | 1:400 |
| Antibody | Anti-mouse CD19-BV480 (clone 1D3) (rat monoclonal) | BD Biosciences | AB_2739509 | 1:400 |
| Antibody | Anti-mouse CD19-PE/Cyanine7 (clone 6D5) (rat monoclonal) | BioLegend | AB_313654 (Cat# 115519); AB_313655 (Cat# 115520) | 1:400 |
| Antibody | Anti-mouse CD45R/B220-FITC (clone RA3-6B2) (rat monoclonal) |
BD Biosciences | AB_394618 | 1:400 |
| Antibody | Anti-mouse CD21/CD35-PE-CF594 (clone 7G6) (rat monoclonal) |
BD Biosciences | AB_2738511 | 1:400 |
| Antibody | Anti-mouse CD138-PE/Cyanine7 Syndecan-1 (clone 281-2) (rat monoclonal) | BioLegend | AB_2562197 (Cat# 142513); AB_2562198 (Cat# 142514) |
1:400 |
| Antibody | Anti-mouse CD27-APC (clone LG.3A10) (Armenian hamster monoclonal) | BD Biosciences | AB_1727455 | 1:400 |
| Antibody | Anti-mouse IgM-APC/Cyanine7 (clone RMM-1) (rat monoclonal) | BioLegend | AB_10690815 (Cat# 406515); AB_10660305 (Cat# 406516) |
1:400 |
| Antibody | CD86-BV510 (clone GL1) (rat monoclonal) | BD Biosciences | Cat# 563077; RRID:AB_2737991 |
1:400 |
| Antibody | Anti-mouse IgM (μ-chain specific) (goat polyclonal M8644) | Sigma | MFCD00145913 | 2 μg/mL |
| Antibody | Anti-mouse IgG1 RMG1-1 (rat monoclonal) | BioLegend | AB_315060 (Cat# 406601); AB_315061 (Cat# 406602) |
2 μg/mL |
| Antibody | Anti-mouse IgG2b (clone R9-91) (rat monoclonal) | BD Biosciences | AB_394834 | 3 μg/mL |
| Antibody | Anti-mouse IgG2c (STAR135) (goat polyclonal) | Bio-Rad | AB_1102666 | 1 μg/mL |
| Antibody | Anti-mouse IgG3 (clone R2-38) (rat monoclonal) | BD Biosciences | AB_394841 | 4 μg/mL |
| Antibody | Anti-mouse IgA (clone C10-3) (rat monoclonal) | BD Biosciences | AB_396541 | 3 μg/mL |
| Antibody | Anti-mouse IgG1 (clone A85-1) (rat monoclonal) | BD Biosciences | AB_393553 | |
| Antibody | Anti-mouse IgG2b (clone R12-3) (rat monoclonal) | BD Biosciences | ||
| Antibody | Anti-mouse AffiniPure anti-mouse IgG, Fcγ subclass 2c specific, (goat polyclonal) |
Jackson | JIR 115-065-208 | |
| Antibody | Anti-mouse IgG3 (clone R40-82) (rat monoclonal) | BD Biosciences | ||
| Antibody | Anti-mouse IgA (clone C10-1) (rat monoclonal) | BD Biosciences | ||
| Commercial assay or kit | HiTrap Protein G HP purification column | Bio-Sciences AB | GE17-0404-01 | |
| Commercial assay or kit | Amicon Ultra-15 Centrifugal Filters | Merck Millipore | 30 kDa MWCO | |
| Commercial assay or kit | Automated chemiluminescence immunoassay | ADVIA Centaur XP | ||
| Commercial assay or kit | ELISA kit | Crystal Chem | ||
| Chemical compound, drug | Lumi-Phos | Lumigen | 33% solution in water |