. 1999 Aug;181(15):4592–4597. doi: 10.1128/jb.181.15.4592-4597.1999

TABLE 1.

Sequence alignment of the substrate binding regions of PrtH and other members of the subtilase family

Protein	Sequence of substrate binding site and adjacent residues
Protein	137–139^a	166^a	156^b	217^b	747–748^a
PrtH	`FSNSDSSASTDSTSII`	`SLGSVS`	`SAGNS`	`MSGTS`	`SLSATKTYYN`
PrtP (SK11)	`FSNSTDSAKTGSATVV`	`SLGSNS`	`SAGNS`	`MSGTS`	`STNRKKTYYN`
PrtP (Wg2)	`FTNSDTSATTGSSTLV`	`SLGSDS`	`SAGNS`	`MSGTS`	`STNLTKTYYN`
PrtB	`FSNNAKNSGAYDDDII`	`SLGSVS`	`SAGNS`	`MSGTS`	`GKEGTKDYYS`
Thermitase^c	`LDN---SGSGTWTAVA`	`SLGGTV`	`AAGNA`	`LSGTS`
Subtilisin BPN′^c	`LGA---DGSGQYSWII`	`SLGGPS`	`AAGNE`	`YNGTS`
	`↑ ↑↑↑↑↑ ↑`	`↑↑↑↑↑↑`	`↑ ↑↑↑`	`↑↑↑↑↑`

Numbering corresponds to that of L. lactis subsp. cremoris SK11 proteinase.

Numbering corresponds to that of subtilisin BPN′.

Alignment is from reference 37, and arrows indicate subtilisin BPN′ and thermitase substrate binding residues.