TABLE 2.

Enzyme activities in Z. mobilis extracts grown on various glucose concentrations^a

Strain	Glucose (M)	GLK Km with glucose (mM)	Sp act (nmol of NADH · mg of protein−1 · min−1) of:
			GLK	G6P	PDC	ADH
ATCC 10988	0.11	0.117 ± 0.011	550 ± 18	160 ± 28	2,135 ± 289	775 ± 35
	0.27	0.110 ± 0.008	770 ± 15	162 ± 10	2,234 ± 250	780 ± 40
	0.55	0.112 ± 0.011	1,050 ± 70	150 ± 14	2,425 ± 210	800 ± 113
CU1Rif2	0.11	0.110 ± 0.006	420 ± 20	145 ± 7	2,335 ± 106	790 ± 98
	0.27	0.090 ± 0.005	370 ± 20	150 ± 12	2,310 ± 107	785 ± 95
	0.55	0.100 ± 0.010	400 ± 15	170 ± 18	2,155 ± 346	790 ± 98
CU1Rif2/pLAFR545	0.11	0.107 ± 0.005	520 ± 15	170 ± 30	2,170 ± 75	830 ± 50
	0.27	0.116 ± 0.009	710 ± 14	165 ± 23	2,170 ± 65	835 ± 35
	0.55	0.114 ± 0.011	910 ± 14	170 ± 22	2,160 ± 45	830 ± 25

^aZ. mobilis cells were grown in complete liquid medium containing 0.11 M glucose, harvested at the mid-logarithmic phase, and incubated in the same medium containing the indicated glucose concentrations for an additional 3 h. Values were estimated from four independent measurements (means ± standard deviations) (4). G6P, glucose-6-phosphate dehydrogenase.