Fig. 5.

APC improves ZAS-induced podocyte apoptosis through ERK1/2/YB-1/PLA2R1 axis. A The expression levels of YB-1 and PLA2R1 were detected by qPCR. B Cell apoptosis was detected after overexpression of YB-1 and PLA2R1 by flow cytometry, and the percentage of apoptosis was calculated. C. TUNEL assay was performed to detect apoptosis in the IMN podocyte model. D Caspase-3 activity kit was used to measure Caspase-3 activity in IMN podocyte model after overexpression of YB-1 and PLA2R1. E The expression levels of APC, p-ERK1/2, ERK1/2, YB-1 and PLA2R1 were detected by western blotting in the IMN podocyte model after overexpression of YB-1 and PLA2R1. The measurement data were presented as Mean ± SD was applied to express data. n = 3, *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars: 100 μm for TUNEL