Figure 5.

Effect of 2HPCD treatment on ZIKV replication in Ae. albopictus cell lines. Cells were treated for 48 h with either PBS or 2HPCD and then infected with ZIKV at an MOI of 1. 72 h post infection total RNA was isolated. qRT-PCR was carried out and data normalised to the mosquito gene RPS17. Tick line represents limit of viral detection on qPCR, therefore anything below represents no virus, each experiment was carried out at least 3 times with a minimum of 2 replicates and combined (*P < 0.05, **P < 0.005 show significant differences in comparisons between the PBS control and each given Wolbachia infection status, Mann–Whitney).