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. 2023 Jul 20;14(7):452. doi: 10.1038/s41419-023-05976-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Scheme illustrating the production of circSLC4A7. B Total RNA was digested with RNase R, followed by qRT-PCR detection of circSLC4A7 expression. SLC4A7 mRNA was detected as the RNase R-sensitive control. C The relative RNA levels of circSLC4A7 and SLC4A7 were analyzed by RT-qPCR after treatment with actinomycin D at the indicated time points. D Cellular localization of circSLC4A7 in AGS and BGC823 cells. The nuclear and cytoplasmic fractions were separated, and the RNA was extracted. CircSLC4A7, U6 and 18 S levels were analyzed by qRT-PCR. The data represent at least three independent experiments. E RNA FISH for circSLC4A7 in AGS and BGC823 cells. Nuclei were stained with DAPI. (scale bar, 50 μm). **P < 0.01 based on the Student t test. All results are from three independent experiments. Data are represented as mean ± SD. At least one representative image was captured.