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. 2023 Jul 20;14(7):452. doi: 10.1038/s41419-023-05976-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Relative expression of circSLC4A7 after its silencing in AGS and BGC823 cells and its overexpression in MGC803 and SGC7901 cells. B Expression of cancer stem cell markers (NANOG, OCT4, SOX2, and CD44) after silencing circSLC4A7 in AGS and BGC823 cells and overexpressing circSLC4A7 in MGC803 and SGC7901 cells, as measured by Western blotting. C Representative images of spheroids formed by AGS-shcircSLC4A7/AGS-Vector cells and MGC803-circSLC4A7/MGC803-Vector cells in suspension cultures. (scale bar, 50 μm). The data are expressed as the number of tumor spheres per well. D Flow Cytometry was adopted to detect the proportions of CD44+/CD54+ cells in AGS, BGC823, MGC803, and SGC7901 cells. The data represent at least three independent experiments. E An immunofluence assay was conducted to detect the expression of cancer stem markers (NANOG and SOX2) after circSLC4A7 knockdown in AGS cells and after overexpression in MGC803 cells. **P < 0.01 based on the Student t test. All results are from three independent experiments. Data are represented as mean ± SD. At least one representative image was captured.