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. 2023 Jul 20;14(7):449. doi: 10.1038/s41419-023-05991-x

Fig. 6. ApoExos transfer RNA to endothelial cells by macropinocytosis.

Fig. 6

A Quantitative measurement of RNA-labeled ApoExo uptake by flow cytometry. Left graph: Time-dependent uptake of RNA-labeled ApoExo by endothelial cells under serum starvation from 1 to 4 h. Right graph: Concentration-dependent uptake of RNA-labeled ApoExos by serum-starved endothelial cells during 1 h. Control (Ctrl) represents cells treated with RPMI serum-free medium. n ≥ 3 for each condition. B Macropinocytosis inhibition suppressed ApoExo RNA uptake by endothelial cells. Quantification of Aphex uptake by serum-starved endothelial cells pretreated for 30 min with 50 µM EIPA or its vehicle (DMSO; Ctrl) followed by treatment for 1 h, as determined by flow cytometry. n ≥ 3 for each condition. Flow cytometry data are expressed as the median fluorescence intensity (MFI) (30,000 events/sample) ± SEM. P values were obtained by unpaired t test (*P < 0.05, **P < 0.01, and ***P < 0.001).