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. 2023 Jul 20;14:4334. doi: 10.1038/s41467-023-40002-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Phylogenetic distribution of PII in 1090 green plants from the One Thousand Plant Transcriptomes Initiative (OTPTI) collection. A simplified phylogenetic tree is displayed on the left and species or other taxonomic names are listed on the right. Clades without PII are labeled in red font. b Colinearity analysis of the PII-containing region in carrot (D. carota), Sc. taccada, and lettuce. PII-containing chromosome 1 in carrot and the corresponding assembled chromosomes in Sc. taccada and lettuce are shown vertically. Red and black lines on the chromosomes indicate the existence and loss of PII, respectively. The gray shade and genome syntelog in the inset show the chromosomal rearrangement between carrot and Sc. taccada. c, d Microsynteny visualization of the colinear chromosome regions between carrot and Sc. taccada (c) and between carrot and lettuce (d). The position of PII in carrot is marked with red arrows. e Bimolecular fluorescence complementation (BiFC) analysis showing the interaction between LsNAGK and PII (DcPII) in vivo. f GST pull-down assays showing the interaction between LsNAGK and PII (DcPII) in vitro. Three times each experiment was repeated independently with similar results. Source data are provided as a Source data file.