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. 2023 Jul 20;14:4334. doi: 10.1038/s41467-023-40002-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, f Number of genes for the Nitrate transporter 2 (NRT2) and NRT3 families in Asteraceae and other terrestrial plant species. **P < 0.01 and ***P < 0.001, as determined by two-tailed Student’s t test. The number (n) of data point for each group was shown below. Data are presented as mean values +/−SD. b, g K_a/K_s value of orthologou_s gene pairs (Sc. taccada versus La. sativa) and paralogous gene pairs (La. sativa versus La. sativa) of clade I NRT2 (d) and clade III NRT3 (i) members. c, h Microsynteny visualization of clade I NRT2 (d) and clade III NRT3 (i) syntelogs in the Sc. taccada and La. sativa genomes. NRT2 and NRT3 orthologous pairs are highlighted with green lines. d, i Simplified phylogenetic tree of NRT2 (d) and NRT3 (i) in Asteraceae species and other species. The genes from the Asteraceae species are marked by dark red, while clades from other species are collapsed. e, j NRT2 (e) and NRT3 (j) expression in different tissues of four representative Asteraceae species. The FPKM values of genes in different tissues are scaled by rows to emphasize the tissue with the highest expression. Source data are provided as a Source data file.