TABLE 3.
RNA fractions isolated from mycobacteria at representative stages of growth
| Sample | Timea | E640 | Stage of growth |
|---|---|---|---|
| M. smegmatis (Lemco broth, 2 hb) | |||
| i | 0 | 0.034 | Inoculum |
| a | 3 | 0.090 | Balanced growth |
| b | 7 | 0.430 | Balanced growth |
| c | 11 | 1.000 | Early stationary |
| d | 16 | 1.350 | Stationary |
| e | 26 | 1.700 | Stationary |
| f | 31 | 1.860 | Stationary |
| g | 36 | 1.700 | Stationary |
| M. smegmatis (Kohn-Harris glucose medium, 23 hb) | |||
| h | 0 | 0.034 | Inoculum |
| j | 20 | 0.070 | Balanced growth |
| k | 36 | 0.240 | Balanced growth |
| l | 56 | 0.590 | Early stationary |
| m | 90 | 0.870 | Stationary |
| n | 121 | 1.230 | Stationary |
| o | 150 | 1.310 | Stationary |
| M. tuberculosis (Dubos medium, 72 hb) | |||
| i | 0 | 0.025 | Inoculum |
| r | 5 | 0.103 | Balanced growth |
| s | 10 | 0.360 | Balanced growth |
| t | 13 | 0.580 | Early stationary |
| u | 22 | 0.680 | Stationary |
| v | 40 | 1.250 | Stationary |
a
Reported in hours for M. smegmatis and in days for M. tuberculosis.
b
Generation time during exponential growth.