FIG. 4.
Titration calorimetry binding isotherm observed upon injecting MgATPγS into NtrC3Ala protein. The titration was carried out at a dimer concentration of NtrC3Ala of 80 μM as described in Materials and Methods. ●, experimental points; ——, fit according to the equation of Wiseman et al. (68); Kd, dissociation constant; ΔH, enthalpy change; n, stoichiometry factor. Deviations represent the fitting error of this single experiment.