Table 1.
Data collection and refinement statistics
| sSLFN11-NTD (1–352, Δ 166–180) (SeMet derivative, PDB: 7FEX) | |
|---|---|
| Data collection | |
| Space group | P6122 |
| Cell dimensions | |
| a, b, c (Å) | 64.99, 64.99, 344.02 |
| α, β, γ (°) | 90.00, 90.00, 120.00 |
| X ray source | |
| Wavelength (Å) | 0.98 |
| Resolution range (Å) | 43.56–2.69 |
| Reflections unique | 22498a |
| R sym b (last shell) | 0.14 (1.15) |
| I/σI (last shell) | 26.33 (3.22) |
| Completeness (%) (last shell) | 99.9 (99.8) |
| Redundancy (last shell) | 34.4 (34.8) |
| CC1/2 (last shell) | 99.90 (88.60) |
| Refinement | |
| Resolution range (Å) | 43.56–2.69 |
| No. reflections, % reflections in cross validation | 22498a, 5.42 |
| R work c/Rfreed (last shell) | 0.22/0.26 (0.33/0.39) |
| Atoms | |
| Non-hydrogen protein atoms | 2549 |
| Protein | 2549 |
| Solvent | 10 |
| B-factors average (Å2) | 66.87 |
| Protein (Å2) | 66.90 |
| Ligands (Å2) | 0 |
| Solvent (Å2) | 59.20 |
| r.m.s.d. | |
| Bond lengths (Å) | 0.003 |
| Bond angles (°) | 0.630 |
| % residues in favored regions, allowed regions, outliers in Ramachandran plot | 96.2, 3.8, 0 |
a Friedel pairs are treated as different reflections.
b R sym = ∑hkl∑j |Ihkl,j - Ihkl|/∑hkl∑jIhkl,j, where Ihkl is the average of symmetry-related observations of a unique reflection.
c R work = ∑hkl ||Fobs(hkl)|-|Fcalc(hkl)||/∑hkl|Fobs(hkl)|.
d R free= the cross-validation R factor for 5% of reflections against which the model was not refined.