Table 1.

Pre-steady-state multiple turnover kinetic parameters of wild-type APE1. Final concentrations of DNA at 100 nM, APE1 at 30 nM. Fold change is calculated from dsDNA values

Substrate	k obs (s−1)	k obs (fold change)	k ss (s−1)	k ss (fold change)
dsDNA	129 ± 24	–	1.7 ± 0.26	–
PTJ	10 ± 1	12.9 ± 2.6	2.7 ± 0.21	1.6 ± 0.3
Rec PTJ	30 ± 4	4.3 ± 0.9	5.9 ± 0.32	3.5 ± 0.6
ssDNA	2 ± 0.2	64.5 ± 12.9	0.15 ± 0.018	11.3 ± 0.4