Skip to main content
. 2023 Jun 7;51(13):6819–6840. doi: 10.1093/nar/gkad449

Figure 2.

Figure 2.

IFI16 displays dynamic exchange with surroundings and IFI16 puncta formation at the nuclear periphery can be inhibited by 1,6-hexanediol. (A) FRAP images showing the recovery of IFI16-GFP after photobleaching. HFF cells stably expressing IFI16-GFP was subjected to FRAP analysis. The red circle indicates the area of photobleaching. (B) Recovery kinetics of IFI16-GFP in (A). Values are means ± SEMs (n = 3). (C) IFI16-GFP stably expressing HFFs were infected with ICP0-RF HSV-1 (MOI = 10, 1 hpi), treated with DMSO or 300 mM 1,6-HD for 10 min and stained for ICP4. Scale bar, 5 μm. (D) Confocal microscopy images showing WT HFFs infected with ICP0-RF HSV-1 (MOI = 10, 1 hpi or 8 hpi), treated with DMSO or 300 mM 1,6-HD for 10 min and stained for endogenous IFI16 and ICP4. Scale bar, 5 μm or 1 μm for inset.