Table 1.
Reagents
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-ICP4, Mouse | Santa Cruz Biotechnology | sc-69809 |
| Anti-alpha-tubulin, Mouse | Sigma Aldrich | T6199 |
| Anti-GAPDH, Rabbit | Cell Signaling | D16H11 |
| Anti-IFI16, Mouse | Santa Cruz Biotechnology | sc-8023 |
| Anti-IFI16, Mouse | Sigma Aldrich | WH0003428M3 |
| Anti-GFP, Mouse | Sigma Aldrich | 11814460001 |
| Anti-NUP153, Mouse | Abcam | ab24700 |
| Anti-histone H3, Rabbit | Abcam | ab1791 |
| Anti-calreticulin, Rabbit | Abcam | ab2907 |
| GFP-Trap Magnetic Agarose | Chromotek | gtma-20 |
| Alexa Fluor secondary antibodies | Thermo Fisher Scientific | A-11001, A-11004, A21141, A21124 |
| Chemicals, inhibitors, and recombinant proteins | ||
| X-tremeGENE HP DNA transfection reagent | Sigma Aldrich | 6366236001 |
| Prolong Diamond Antifade Mountant | Thermo Fisher Scientific | P36961 |
| Immobilon-FL PVDF membranes | Millipore Sigma | IPFL00010 |
| TCEP | ThermoFisher Scientific | 77720 |
| S-TRAP Micro Spin Columns | Protifi | CO2-micro-10 |
| DAPI | Thermo Fisher Scientific | 62248 |
| AT7519 | Cayman Chemical | 16231 |
| 1,6-Hexanediol | Sigma Aldrich | 240117 |
| Recombinant cGAS | Cayman Chemical | 22810 |
| Recombinant IFI16 | OriGene | TP302193 |
| TrueCut Cas9 Protein v2 | Invitrogen | A36498 |
| Recombinant Cdk2/Cyclin E | Sigma Aldrich | 14–475 |
| Recombinant DNA-PK | Life Technologies Corporation | PV5866 |
| Recombinant GSK3 beta | Abcam | ab60863 |
| Critical commercial assays, kits and reagents | ||
| BCA Assay | Thermo Fisher Scientific | 23225 |
| Roche In Situ Cell Death Detection Kit – Fluorescein TUNEL Assay | Sigma Aldrich | 11684795910 |
| Alexa Fluor™ 488 Protein Labeling Kit | Thermo Fisher Scientific | A10235 |
| HiScribe® T7 High Yield RNA Synthesis Kit | New England BioLabs | E2040S |
| Applied Biosystems PowerUp SYBR Green Master Mix | Thermo Fisher Scientific | A25778 |
| Oligonucleotides | ||
| Cy5-VACV70mer F | IDT | 5′- CCATCAGAAAGAGGTTTAATATTTTTGTGAGACCATCGAAGAGAGAAAGAGATAAAACTTTTTTACGACT |
| Cy5-VACV70mer R | IDT | 5′- AGTCGTAAAAAAGTTTTATCTCTTTCTCTCTTCGATGGTCTCACAAAAATATTAAACCTCTTTCTGATGG |
| isg54 qPCR primer F | IDT | 5′-ACGGTATGCTTGGAACGATTG |
| isg54 qPCR primer R | IDT | 5′- AACCCAGAGTGTGGCTGATG |
| isg56 qPCR primer F | IDT | 5′-AAGGCAGGCTGTCCGCTTA |
| isg56 qPCR primer R | IDT | 5′-TCCTGTCCTTCATCCTGAAGCT |
| icp4 qPCR primer F | IDT | 5′-GCGTCGTCGAGGTCGT |
| icp8 qPCR primer R | IDT | 5′-CGCGGAGACGGAGGAG |
| icp4 qPCR primer F | IDT | 5′-GTGGTTACCGAGGGCTTCAA |
| icp8 qPCR primer R | IDT | 5′-GTTACCTTGTCCGAGCCTCC |
| ul5 qPCR primer F | IDT | 5′-TCGTGAGGTCCAAAATCACC |
| ul5 qPCR primer R | IDT | 5′-CGACCCATCAACACCATCTT |
| ul30 qPCR primer F | IDT | 5′- GCGAAAAGACGTTCACCAAG |
| ul30 qPCR primer R | IDT | 5′-CGGAGACGGTATCGTCGTAA |
| GAPDH qPCR primer F | 5′-TTCGACAGTCAGCCGCATCTTCTT | |
| GAPDH qPCR primer R | 5′-CAGGCGCCCAATACGACCAAATC | |
| MISSION(R) siRNA Universal Negative Control #1 | Sigma Aldrich | Proprietary |
| MISSION(R) siRNA Universal Negative Control #1 | Sigma Aldrich | Proprietary |
| MISSION(R) siRNA targeting human CDK2 | Sigma Aldrich | Proprietary |