Table 2.
Plasmids used in this study
| Name | Description | Source |
|---|---|---|
| pTKB612 (pC4002) | EFT2-6xHis LEU2 CEN | (61) |
| pC5533 | EFT2-6xHis-R180S LEU2 CEN | This study |
| pC5111 | YCplac33-DPH2 URA3 CEN | This study |
| pC6725 | -1 ribosomal frameshifting site of SARS-CoV-2 in LacZ gene, URA3 CEN (from pJD204.-1) | This study |
| pC6726 | In-frame control for pC6725 | This study |
| pC6727 | -1 ribosomal frameshifting site of HIV in LacZ gene, URA3 CEN (from pJD204.-1) | This study |
| pC6728 | In-frame control for pC6727 | This study |
| pJD204.-1 (pC5610) | URA3 CEN (-1 ribosomal frameshifting site of L-A virus in N-terminal region of LacZ gene) | (63) |
| pJD204.+1 (pC5609) | URA3 CEN (+1 ribosomal frameshifting site of Ty1 in LacZ gene) | (63) |
| pJD204.0 (pC5608) | URA3 CEN (in-frame control for pC5610 and pC5609) | (63) |
| pC6500 | LacZ gene -1 frame to AUG start codon (no programmed frameshifting site) URA3 CEN (from pJD204.-1) | This study |
| pC6501 | LacZ gene + 1 frame to AUG start codon (no programmed frameshifting site) URA3 CEN (from pJD204.-1) | This study |
| pC6502 | LacZ gene 0 frame to AUG start codon (in-frame control for C6500 and C6501) URA3 CEN | This study |
| pJD375 (pC3752) | pYDL-control (Firefly luciferase 0 frame to Renilla luciferase URA3 CEN) | (25) |
| pC6499 | Firefly luciferase -1 frame to Renilla luciferase in pJD375 URA3 CEN | This study |
| pC6498 | Firefly luciferase + 1 frame to Renilla luciferase in pJD375 URA3 CEN | This study |
| pC3752 | Firefly luciferase 0 frame to Renilla luciferase in pJD375 URA3 CEN (in-frame control for C6499 and C6498) | This study |
| pEKD0209 (pC4310) | pPGK1-Renilla-GluGAA10-firefly URA3 2μ | (26) |
| pC5380 | Renilla ΔG62 reporter URA3 2μ (Deletion of firefly luciferase gene from pEDK0209) | This study |
| pC5379 | In-frame control for pC5380 | This study |
| pC6205 | pSynoYAC0-MDN1-WT HIS3 CEN (pSynoYAC0 from LifeSct) | This study |
| pC6160 | pSynoYAC0-MDN1-LS HIS3 CEN (Low out-of-frame Stop codons, synthesized by LifeSct, Rockville, Maryland) | This study |
| p2luc (pC5150) | Dual (Renilla-Firefly) luciferase reporter | (64) |
| pC6615 | -1 ribosomal frameshifting site from SARS-CoV-2 in p2luc | This study |
| pC6616 | In-frame control for pC6615 | This study |
| pC6619 | -1 ribosomal frameshifting site from HIV in p2luc | This study |
| pC6620 | In-frame control for pC6619 | This study |
| pRS415-REA1 (pC6334) | pRS415 REA1 LEU2 CEN | (62) |
| pRS415-rea1-7 (pC6335) | pRS415 rea1-7 LEU2 CEN | (62) |
| pRS415-rea1-21 (pC6336) | pRS415 rea1-21 LEU2 CEN | (62) |
| pTAP-REA1 (pC6337) | pTAP-REA1 LEU2 CEN | (62) |
| pTAP-rea1-DTS (pC6339) | pTAP-rea1-DTS LEU2 CEN | (62) |
| pMN3 (pC6157) | GFP with modified GAL1 promoter (GCGTGGGCG), URA3 CEN | (24) |
| pC6310 | Ubi-DTA in pMN3 | This study |
| pC4464 | pET15b 6xHis-DTA | Gerald Marsischky & R. John Collier, Harvard Medical School |