Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 13;51(13):6770–6783. doi: 10.1093/nar/gkad505

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Stabilization of the NHEJ machinery at DSBs is promoted by phosphorylation of DNA-PK_cs at T4102. (A) Phosphorylation of DNA-PK_cs at T4102 is not required for the recruitment of Ku80 to laser-induced DSBs. Recruitment of GFP-Ku80 was monitored in CHO V3 cells stably expressing FLAG-tagged DNA-PK_cs WT, T4102A and T4102D. The data are presented as mean ± SEM with P-values calculated via Student's t-test. n.s., not significant. (B-D) Ablating T4102 phosphorylation attenuates the recruitment of GFP-XRCC4 (B), GFP-XLF (C) and GFP-PNKP (D) to laser-generated DSBs. Relative fluorescent intensity of GFP-tagged XRCC4 (B), XLF (C) and PNKP (D) in CHO V3 cells with FLAG-tagged DNA-PKcs WT, T4102A and T4102D are presented as mean ± SEM and significance was assessed via a Student's t-test. The bar indicates 5 μm.