Fig. 6. The effects of anti-TIMP-1 antibody treatment in a mouse model of chronic allergic asthma. (A) Airway hyperresponsiveness. (B) Total cells and eosinophils in the BALF. The levels of (C) EDN, (D) dsDNA, and (E) TIMP-1 in the BALF (n = 5 for each group). Data are presented as mean ± standard deviation. (F) Lung histology stained with H&E and MT staining. Immunofluorescence staining for DAPI (nuclear, blue), MBP (turquoise), and CD63 (yellow). Scale bar, 200 µm. (G) The expressions of MBP and E-Cad in the lung tissues.
BALF, bronchoalveolar lavage fluid; DAPI, 4’,6-diamidino-2-phenylindole; Dex, dexamethasone; EDN, eosinophil-derived neurotoxin; H&E, hematoxylin and eosin; MT, Masson’s trichrome; MBP, major basic protein; RL, resistance to airflow across the lung; OVA, ovalbumin; TIMP-1, tissue inhibitor of metalloproteinase-1; E-Cad, E-cadherin; PBS, phosphate buffered saline.
*P < 0.05, **P < 0.01, and ***P < 0.001 by one-way analysis of variance with the Bonferroni post hoc test.