Table 2.
Summary of symptom development and tlaRNA systemic movement in the various single and mixed virus infections established in Nicotiana benthamiana.
| Single infection | Double infection | Triple infection | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Infecting viruses | TuYV | CMoV | Gamma | Sigma | ST9 | TuYV + Gamma | TuYV + Sigma | TuYV + ST9 | TuYV + CMoV | CMoV + Gamma | CMoV + Sigma | CMoV + Gamma | TuYV + CMoV + Gamma | TuYV + CMoV + Sigma | TuYV + CMoV + ST9 | |
| Symptoms | No | No | No | No | No | No | No | No | Yes | No | Yes | Yes | Yes | Yes | Yes | |
| tlaRNA systemic movement | - | - | 0/10 | 0/8 | 0/8 | 0/10 | 0/7 | 3/8 | - | 4/10 | 8/8 | 8/8 | 4/10 | 8/8 | 8/8 | |
| No. of plants infected | TuYV | 22/23 | - | - | - | - | 10/10 | 7/7 | 8/8 | 22/23 | - | - | - | 10/10 | 8/8 | 8/8 |
| CMoV | - | 21/23 | - | - | - | - | - | - | 23/23 | 9/10 | 8/8 | 8/8 | 10/10 | 8/8 | 8/8 | |
| tlaRNA | - | - | 10/10 | 8/8 | 8/8 | 10/10 | 7/7 | 8/8 | - | 10/10 | 8/8 | 8/8 | 10/10 | 8/8 | 8/8 | |
The top row of the table indicates the infecting virus(es), the second row indicates whether symptoms were observed in plants infected with the respective single and co-infection treatments, and the third row indicates the number of plants in which the co-infecting tlaRNA could be detected in the upper non-inoculated leaves. The bottom three rows of the table indicate the infection rates (number of plants infected/number of plants inoculated) for all of the viruses in each treatment.