Table 1.
Affinity and inhibitory activity of a panel of anti-IL-13 VHHs.
| VHH | IL-13 KD (µM) | IL-13 IC50 (µM) |
|---|---|---|
| 201 | 0.9 ± 0.1 | 37.7 ± 1.1 |
| 204 | 2.6 ± 0.5 | 26.2 ± 1.0 |
| 209 | 2.2 ± 0.2 | 67.8 ± 1.0 |
| 210 | 0.04 ± 0.008 | 0.2 ± 0.001 |
| 214 | 4.8 ± 0.9 | 153.8 ± 1.1 |
| 227 | 5.5 ± 0.9 | 63.5 ± 1.1 |
| 228 | 0.9 ± 0.1 | 56.0 ± 1.1 |
| 229 | 0.2 ± 0.08 | 11.4 ± 1.2 |
| 234 | 4.1 ± 0.6 | 49.6 ± 1.1 |
| 235 | 3.6 ± 0.3 | 74.3 ± 1.2 |
| 238 | 0.2 ± 0.03 | 1.7 ± 0.01 |
| 245 | 0.2 ± 0.01 | 3.7 ± 0.01 |
| 251 | 2.2 ± 0.5 | 6.9 ± 1.1 |
| 264 | 3.9 ± 0.5 | 14.5 ± 1.1 |
| 281 | 0.4 ± 0.02 | 34.3 ± 1.1 |
KD values are reported with the standard error obtained from fitting of the binding curves in Prism. The IC50 values are reported as the mean ± standard deviation from two independent experiments.