Figure 6.
Functional activities of Pfs25-EPA and Pfs230D1-EPA are dependent on the presence of complement
Standard membrane feeding assays (SMFA) were performed to evaluate (A) Pfs25 and (B) Pfs230D1, and (C) Pfs25+Pfs230D1 vaccine-induced transmission-reducing activity (oocyst reduction in mosquito midguts). Sera from all animals within vaccine dosing groups (see Figure 4) were pooled for feeding assays. All sample pools were run in the same assay; baseline points are identical in all panels. Assays were run in either the presence or absence of complement. Data are presented as oocyst counts on a log10 scale; an offset of +1 was applied to all values to account for raw values of 0. Lines represent means with error bars indicating 95% CI. Differences in SMFA oocyst counts between adjuvants were analyzed by Kruskal-Wallis with Dunn’s test for multiple comparisons. Significant Dunn’s adjusted p-values are presented as asterisks: ∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p ≤ 0.0001.
See also Figures S11 and S12.