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. 1999 Aug;181(15):4661–4664. doi: 10.1128/jb.181.15.4661-4664.1999

FIG. 2.

FIG. 2

Physical and genetic map of loci required for the secretion of protease, lipase, and PLC in B. pseudomallei. (A) Genetic locus containing 26 distinct Tn5-OT182 integrations in B. pseudomallei secretion mutants. (B) Genetic locus containing three distinct Tn5-OT182 integrations in B. pseudomallei secretion mutants. The horizontal lines represent B. pseudomallei chromosomal DNA, and the locations of relevant restriction endonuclease sites are shown (B, BamHI; Bg, BglII; E, EcoRI). The exact locations of Tn5-OT182 integrations in the secretion mutants are shown by vertical lines extending above and below the chromosomal DNA. The promoterless lacZ gene of Tn5-OT182 integrations is oriented from left to right (5′ to 3′) in the secretion mutants designated by vertical lines extending above the chromosomal DNA and from right to left (5′ to 3′) in the secretion mutants designated by vertical lines extending below the chromosomal DNA. The locations and directions of the transcription of the genes are represented by arrows, and the gene names are shown below them. A scale (in kilobases) is shown at the bottom.