Skip to main content
. 2023 Jul 7;14:1177324. doi: 10.3389/fimmu.2023.1177324

Figure 4.

Figure 4

T cell response against TBEV NS1. (A) For IFN-γ ELISpot, mouse splenocytes were restimulated overnight with TBEV NS1 peptide pools NS11-183 and NS1173-352. Mean values with SD are shown as IFN-γ spot-forming cells (SFC) per one million splenocytes after background subtraction. For statistical analysis, unpaired t-test was used (**p<0.01). (B) Calculated IFN-γ SFC per million splenocytes for total NS1 (NS11-183 + NS1173-352). One-way ANOVA with Tukey’s multiple-comparison test was used for statistical analysis. Significances are only shown for NS1-vaccine groups (ns = not significant, ** p<0.01, **** p<0.0001). (C–G) Flow cytometric analysis of mouse splenocytes. Frequency of CD3+ subpopulations gated on CD4+ (C, D) and CD8+ (E–G) T cells positive for CD69, IFN-γ and Granzyme B (GrzB) upon restimulation with NS11-183 (black circle) or NS1173-352 (unfilled square) (n = 4). Bars represent mean with SD. Data is shown after background subtraction.