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Dose titration of amphiphilic xenopeptides and logD7.4 correlation with eGFP knockout efficiencies. (A) eGFP knockout efficiency in HeLa eGFP/tub cells after 48 h treatment with Cas9 RNP nanocarriers at a series of RNP concentrations between 0.1 and 100 nM. Data are presented as mean ± SD (n = 3). (B) Plot of eGFP knockout EC50 values versus logD7.4 values of each xenopeptide series.
