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. 2023 Jun 24;34(7):1271–1281. doi: 10.1021/acs.bioconjchem.3c00163

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© 2023 The Authors. Published by American Chemical Society

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UHPLC chromatograms at 280 nm for tailored labeling experimental conditions. Full chromatograms are provided in the Supporting Information, Oligopeptide Labeling. (A) Highly selective CBI 12 was incubated for 3 h or with a 10-fold excess to achieve complete conversion of UP1. Full chromatograms are provided in the Supporting Information, Figure S25. (B) When highly reactive CBT 83 was incubated in an equimolar amount with the oligopeptides, labeling of residues other than the N-terminal Cys was significantly reduced compared with 2-fold excess of the compound. Full chromatograms are provided in the Supporting Information, Figure S26.