Table 2. Minimum Inhibitory Concentrations (MIC) of Tobramycin-Bac7 Conjugates toward Bacterial Reference and Clinical Strainsa.
| MICb (μM) |
|||||
|---|---|---|---|---|---|
| Bacterial strain | Tob | mTob-Bac7(1–35)[Cys36]-OH | mTob-Bac7(1–15)[Cys16]-NH2 | Bac7(1–35)[Cys36ALK]-OH | Bac7(1–15)[Cys16ALK]-NH2 |
| E. coli BW25113 | 4 | 1 | 4 | 2 | 8 |
| E. coli BW25113ΔsbmA | 16 | 2 | 4 | 8 | >32 |
| A. baumannii ATCC 10606 | 4 | 2 | 2 | 2 | 16 |
| S. enteritidis ATCC 14028 | 8 | 1 | 4 | 1 | 32 |
| P. aeruginosa ATCC 27853 | 1 | 1 | 4 | 32 | >32 |
| P. aeruginosa PA01 | 1 | 1 | 2 | 16 | >32 |
| P. aeruginosa PA05 | 2 | 1 | 2 | 4 | >32 |
| P. aeruginosa PA10 | >32 | 2 | 4 | >32 | >32 |
| P. aeruginosa PA21 | >32 | 2 | 4 | >32 | >32 |
| P. aeruginosa PA22 | 0.5 | 1 | 1 | 4 | >32 |
| P. aeruginosa PA35 | >32 | 1 | 4 | 1 | 32 |
a
Native tobramycin and Bac7 fragments were used for comparison.
b
All experiments were carried out in triplicate and repeated three times, inoculating 2.5 × 105 CFU/mL bacteria in 100% MH broth at 37 °C for 18 h. MIC values were visually evaluated as the lowest concentration at which bacterial growth was inhibited (no turbidity/deposit on bottom of wells).