Detection and qPCR validation of the differentially expressed ferroptosis-related genes.
(A, B) Heatmaps of the top differentially expressed genes related to ferroptosis from RNA sequencing in the CSCI_4w (A) and CSCI_8w groups (B). The color scale indicates the relative gene expression level. (C) Quantitative reverse transcription-polymerase chain reaction validations of the differentially expressed genes characterized by RNA sequencing. *P < 0.05, ***P < 0.001, vs. sham group; ###P < 0.001, vs. CSCI_4w group (Student’s t-test). Sample size: sham, n = 4; CSCI_4w, n = 4; CSCI_8w, n = 3. ARNTL, GPX4, MafG, FAR1, PTEN were the top differentially expressed ferroptosis-related genes. ARNTL: Aryl hydrocarbon receptor nuclear translocator-like protein 1; FAR1: fatty acyl-CoA reductase 1; GPX4: glutathione peroxidase 4; MafG: MAF BZIP transcription factor G; PTEN: phosphatase and tensin homolog; qPCR: quantitative polymerase chain reaction.