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. 2023 Mar 15;18(11):2482–2488. doi: 10.4103/1673-5374.371378

Figure 4.

Figure 4

Detection and qPCR validation of the differentially expressed ferroptosis-related genes.

(A, B) Heatmaps of the top differentially expressed genes related to ferroptosis from RNA sequencing in the CSCI_4w (A) and CSCI_8w groups (B). The color scale indicates the relative gene expression level. (C) Quantitative reverse transcription-polymerase chain reaction validations of the differentially expressed genes characterized by RNA sequencing. *P < 0.05, ***P < 0.001, vs. sham group; ###P < 0.001, vs. CSCI_4w group (Student’s t-test). Sample size: sham, n = 4; CSCI_4w, n = 4; CSCI_8w, n = 3. ARNTL, GPX4, MafG, FAR1, PTEN were the top differentially expressed ferroptosis-related genes. ARNTL: Aryl hydrocarbon receptor nuclear translocator-like protein 1; FAR1: fatty acyl-CoA reductase 1; GPX4: glutathione peroxidase 4; MafG: MAF BZIP transcription factor G; PTEN: phosphatase and tensin homolog; qPCR: quantitative polymerase chain reaction.