Figure 7.
Correlation between GPX4 and MafG expression, and the MafG-Nrf2 interaction after chronic CSCI.
(A, B) Western blotting of GPX4 and MafG was significantly decreased at week 4 but increased at week 8 following chronic CSCI, compared with that of the sham group. (C) Pearson correlation analysis indicated a significant positive correlation between MafG and GPX4 expression (R2 = 0.879; P < 0.001). (D, E) Immunoprecipitation (IP) assay demonstrating the interaction between MafG and Nrf2, which was attenuated at 4 weeks and significantly increased at 8 weeks following spinal cord compression. *P < 0.05, **P < 0.01, vs. sham group; ###P < 0.001, vs. CSCI_4w group (Student’s t-test). Data are presented as the mean ± standard deviation in B and E. Sample sizes: sham, n = 4; CSCI_4w, n = 4; and CSCI_8w, n = 3. CSCI: Compressive spinal cord injury; GPX4: Glutathione peroxidase 4; MafG: MAF BZIP transcription factor G; Nrf2: nuclear factor erythroid 2-related factor 2.