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Sirt6 expression in the PrL after chronic sleep deprivation treatment via immunohistochemistry (A and B) and western blot assay (C and D).
Chronic sleep deprivation reduced Sirt6 expression (green, Alexa Fluor 488) in neurons (marked by NeuN, red, Cy3). Data are presented as mean ± SEM, and were analyzed by Student’s t-test. White arrows indicate Sirt6 expression in neurons. CSD: Chronic sleep deprivation; DAPI: 4′,6-diamidino-2-phenylindole; PrL: prelimbic cortex; Sirt6: Sirtuins 6.
